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Today, let’s say good fragment of DNA had a grams-C content out-of 31%

Today, let’s say good fragment of DNA had a grams-C content out-of 31%

The low G-C content informs us that this piece of DNA has actually an effective lowest melting section in fact it is far more available to polymerases. Chargaff’s laws allow us to determine your DNA should be 15% guanine and you can 15% cytosine.

I also be aware that other nucleotide articles during the this new DNA must be composed of adenine and you will thymine (as the DNA have two types of nucleotide bonds). Ergo, the fresh new portion of mutual adenine and you will thymine content need to be 70%, otherwise 35% for every single.

We currently know the DNA fragment includes 15% guanine, 15% cytosine, 35% adenine, and you can 35% thymine. If we range from the proportions of purine nucleotides together with her and you can pyrimidine nucleotides with her as with Chargaff’s rule, we obtain 50% purine posts and fifty% pyrimidine articles: ultimately causing a 1-to-step 1 ratio, exactly as Chargaff explained.
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